Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

The enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. enantioseparation a group fungicides (sulconazole, bifonazole, triadimefon triadimenol) using supercritical fluid chromatography was achieved this work. For goal, four chiral columns based on polysaccharide derivatives, as well effect chromatographic parameters such temperature, type percentage organic modifier (methanol, ethanol isopropanol), were thoroughly investigated. inversion elution order enantiomers result change stationary phase or also evaluated by employing circular dichroism detector. best conditions, terms enantioresolution analysis time, obtained with Lux® Cellulose-2 column isopropanol modifier.